Ranking Value: 352
| Champion | LEXINGTON FC PREMIER (KY) |
| Finalist | TENNESSEE UNITED SC 96 RED (TN) |
| Semi-Finalist | MURFREESBORO SC STRIKERS PREMIER '96 (TN) |
| Qtr-Finalist | AFFC U14 GIRLS (KY) |
| - | CLARKSVILLE IMPACT 96 LADY IMPACT CLARKSVILLE (TN) |
| Teams | MP | W | D | L | GF | GA | PTS |
|---|---|---|---|---|---|---|---|
| AFFC U14 GIRLS (KY) | 4 | 1 | 1 | 2 | 1 | 4 | 12 |
| CLARKSVILLE IMPACT 96 LADY IMPACT CLARKSVILLE (TN) | 4 | 0 | 1 | 3 | 2 | 6 | 5 |
| LEXINGTON FC PREMIER (KY) | 4 | 4 | 0 | 0 | 9 | 2 | 35 |
| MURFREESBORO SC STRIKERS PREMIER '96 (TN) | 4 | 1 | 2 | 1 | 3 | 2 | 17 |
| TENNESSEE UNITED SC 96 RED (TN) | 4 | 2 | 0 | 2 | 4 | 5 | 18 |
| Friday, March 19, 2010 | ||||
|---|---|---|---|---|
| 7:00 PM | MURFREESBORO SC STRIKERS PREMIER '96 | 2 - 0 | TENNESSEE UNITED SC 96 RED | |
| Saturday, March 20, 2010 | ||||
| 8:00 AM | LEXINGTON FC PREMIER | 3 - 0 | AFFC U14 GIRLS | |
| 1:00 PM | LEXINGTON FC PREMIER | 3 - 1 | TENNESSEE UNITED SC 96 RED | |
| 1:00 PM | MURFREESBORO SC STRIKERS PREMIER '96 | 1 - 1 | CLARKSVILLE IMPACT 96 LADY IMPACT CLARKSVILLE | |
| 6:00 PM | CLARKSVILLE IMPACT 96 LADY IMPACT CLARKSVILLE | 0 - 1 | AFFC U14 GIRLS | |
| Sunday, March 21, 2010 | ||||
| 8:00 AM | MURFREESBORO SC STRIKERS PREMIER '96 | 0 - 0 | AFFC U14 GIRLS | |
| 8:00 AM | CLARKSVILLE IMPACT 96 LADY IMPACT CLARKSVILLE | 0 - 2 | TENNESSEE UNITED SC 96 RED | |
| 11:30 AM | LEXINGTON FC PREMIER | 2 - 1 | CLARKSVILLE IMPACT 96 LADY IMPACT CLARKSVILLE | |
| 11:30 AM | TENNESSEE UNITED SC 96 RED | 1 - 0 | AFFC U14 GIRLS | |
| 2:00 PM | LEXINGTON FC PREMIER | 1 - 0 | MURFREESBORO SC STRIKERS PREMIER '96 | |